M.bovis Genetic Diversity

M.bovis Genetic Diversity Mycoplasmabovis pneumonia is an epidemic worldwide. To understand M.bovis genetic diversity would help develop novel measures to control this disease. Therefore this study was aimed to determine genotype distribution of Chinese strains and the potential global evolution. Firstly three available methods including two M. bovis multilocus sequence typing (MLST) schemes MLST-1 and MLST-2 and pulsed field gel electrophoresis (PFGE) were comparatively used for 44 Chinese strains and M. bovis type strain PG45 originated fromUSA. The results showed a high genetic homogeneity of Chinese isolates. By MLST-1, 43 of 44 (97.7%) Chinese isolate being ST-10, while 1 of 44 ST-34. The MLST-2 scheme clustered 44 Chinese isolates into two sequence types, ST-10 43 of 44 (97.7%) and 1 of 44 ST-32. PFGE clustered 42 of 44 (95.5%) into PT-I. The discrimination index was highest for PFGE (D = 0.160), while both MLST schemes have similar discrimination power (D = 0.110). The agreement rate among three typing methods is 95.4%à ¯Ã‚ ¼Ã‹â€ 95% CIà ¯Ã‚ ¼Ã… ¡84.2%, 99.4%à ¯Ã‚ ¼Ã¢â‚¬ °. The type strain PG45 gave a unique type by all three methods. Additionally, MLST-2 scheme was used to analyze 8 Australia and 8 Israeli isolates. The results showed 8 Israeli strains represent three STs with ST-10 as the most dominant type comprising 50% of the strains, ST-20 (n=2) and ST-28 (n=2). The 8 Australian isolates showed two sequence types ST-10 (n=7) and another sequence type ST-41 (n=1) identified firstly here. The assay of evolutionary relationship by geoBURST Minimum spanning tree (MST) of 60 isolates typed in this study and 207 isolates of 11 countries from the MLST-2 database. It was revealed that similar dominant clone (ST-10 in CC 3) exists in China, Israel, Australia and United States. This may be related to global livestock movements. In conclusion, we firstly demonstrated the remarkable clonality of M. bovis in China and the dominant ST-10 might originate from a common global source. Key words: Mycoplasma bovis; molecular epidemiology; multilocus sequence typing (MLST); pulsed field gel electrophoresis (PFGE); cattle; evolution. Mycoplasma bovis (M.bovis) is the main causative pathogen of bovine mycoplasmosis worldwide such as in North America, Europe (Nicholas and Ayling, 2003), China (Shi et al., 2008), Australia (Morton et al., 2014) and Israel (Lysnyansky et al., 2016). It results in substantial economic losses to producers by causing M.bovispneumonia and mastitis in beef and dairy cattle. M. bovis was first isolated in 1961 in USA from cattle mastitis milk (Hale et al 1962) and has long been considered a player in bovine respiratory diseases (BRD) since 1976 (Thomas et al., 1986). It then appears to have spread via animal movements to, amongst many countries (Nicholas 2002). Today, infection occurs in most European countries and throughout the world. It was estimated that the economic loss caused by M.bovisin United States was up to $108 million per year. In Europe, M.bovis pneumonia constitutes about 30% of calf respiratory diseases (Nicolas and Ayling, 2003; Maunsell et al., 2011). As the prevalence o f M. bovis associated diseases varies widely across the world, there are important trade implications and a pressing need to monitor cattle for M. bovis. However, to date, there are large gaps in our understanding evolutionary relationships of this pathogen isolates between different countries and globally. In China the first M. bovis mastitis was described in 1983 (Chen et al., 1983) and first M. bovis pneumonia in 2008. Since then reports of M. bovis pneumonia and mastitis outbreaks have been frequently described (Shi et al., 2008; Peng et al., 2011). M. bovis pneumonia is characterized by severe respiratory distress, high fever and at postmortem lung lesions including carnification, extensive caseo-necrotic or suppurative foci in the lungs. M.bovis pneumonia caused over 80% morbidity and between 10% to 60% mortality in calves and stockers newly introduced into beef feedlots (Shi et al., 2008). A major contributing factor to this disease is the stress caused by the long distance transportation of calves and stockers between the feedlots and farms (Shi et al., 2008). The disease is difficult to control with chemotherapy, and vaccination would be an ideal alternative approach. An insight of the genetic diversity and population structure of M. bovis would assist in the development of novel vaccines, as well as gaining an insight into evolutionary trends. A variety of molecular typing methods have been used for epidemiological characterization of M. bovis strains including random amplified polymorphic DNA (RAPD) analysis (Butler et al., 2001), amplified fragment length polymorphism (AFLP) analysis (Kusiluka et al., 2000; Soehnlen et al., 2012), pulsed field gel electrophoresis (PFGE) (Pinho et al., 2012; Arcangioli et al., 2012), insertion sequence (IS) typing (Miles et al., 2005; Aebi et al., 2012) and multilocus variable number tandem repeats (VNTR) analysis (Pinho et al., 2012; Spergser et al., 2013). In addition, three multi-locus sequence typing (MLST) schemes were recently developed to study population structure, evolution and spread of this pathogen (Manso-Silvan et al., 2012;Register et al., 2015; Rosales et al., 2015). The MLST scheme developed by Manso-Silvan et al. (2012) is based on four housekeeping genes fusA, gyrB, lepAand rpoB and showed a discrimination index of 0.833, while improved MLST scheme have been developed by Rosales et al.2015) here after referred as MLST-1 scheme; and by Register et al. (2015) here after referred as MLST-2 scheme. Both schemes use seven housekeeping genes but they only have one gene in common and therefore theire discrimination power higher than the Manso-Silvan scheme. In the present study, it was aimed to firstly evaluate the three methods MLST-1 and MLST-2 schemes and conventional PFGE by comparing the results in typing 44 Chinese M.bovis isolates, secondly assess the genetic diversity and population structure of M. bovis strains isolated in period of 2007 2014 by using the type strain PG45 as the control., and thirdly explore the evolutionary relationship of Chinese M.bovis isolates with globally diverse isolates. Material and Methods Mycoplasma bovis isolates M. bovis Chinese isolates (n=44) were obtained during 2008 to 2014 from nine Chinese provinces: Hubei (n=25), Anhui (n=1), Fujian (n=2), Hunan (n=1), Jiangxi (n=3), Henan (n=8), Inner Mongolia (n=1), Guangzhou (n= 2) and Shandong (n=1). These M. bovis isolates were mostly from lungs in cases of pneumonia (n=41); together with other sources such as milk with mastitis (n=2); throat swab in case of pneumonia (n=1) and fluid of joint with arthritis (n=1). The M. bovis type strain PG45 was purchased from American Type Culture Collection (ATCC 25523) and also used in this study. DNA samples from 8 Israeli M. bovis isolates were kindly offered by Prof. Dr. Inna Lysnyansky from Kimron Veterinary Institute, Israel, collected during 2013-2014 from pneumonia (n=6), stillbirth (n=1) and arthritis (n =1) in seven regions namely Gilboa (n=1), Beer Tuvia (n=3), Hevel Eilot (n=1), Eshkol (n=1), Jerusalem (n=1), Mateh Yehuda (n=1) and EmekYizrael (n=1). In addition, eight whole genome sequences of Australian M.bovis isolates were retrieved from GenBank representing mastitis, (n=4), lungs (n=1), nose swab (n=1), joint fluid (n=1) and semen culture (n=1) in five regions namely New south Wales (n=2), Queensland (n=1), Tasmania (n=3), South Australia (n=1) and Victoria (n=1) with accession no. SAMN05444185, SAMN05444199, SAMN05444228, SAMN05444239, SAMN05444243, SAMN05444247, SAMN05444250, SAMN05444261) included in this study (Table 1). Growth conditions, species identification and DNA extraction M.bovis isolates were confirmed by species-specific PCR as previously described (Subramaniam et al., 1998). The M.bovis samples were grown in PPLO broth (Difco) supplemented with 0.5% (w/v) sodium pyruvate (Biosharp, China), 0.09% (w/v) yeast extract (BD Biosciences, San Jose, CA, USA)à ¯Ã‚ ¼Ã…’0.004% (w/v) phenol red, 1% (v/v) 10- minimum essential medium (MEM) (Sigma-Aldrich, Saint Louis, MO, USA), 20% (v/v) Hyclone donor horse serum (Invitrogen, Carlsbad, CA, USA) and penicillin G 80,000 IU/100 mL and the final pH was adjusted to 7.6(Khan et al., 2016). DNA from each isolate was extracted using the genomic DNA extraction kit (Tiangen, Beijing, China). Multilocus sequence typing (MLST) MLST-1 scheme is based on a partial sequencing of dnaA, metS, recA, tufA, atpA, rpoD and tkt genes (Rosales et al., 2015); For MLST-1 scheme, 44 Chinese isolates and American type strain PG45. The PCR amplification conditions for MLST-1 were used as previously described (Rosales et al., 2015); after amplification, PCR products were further purified and sequenced using PCR Products Extraction Kit (Magnetic Beads) (Enriching Biotechnology, LTD, Wuhan, China) and sequenced. Sequencing reactions were performed by the commercial company (Tianyi Hui Yuan Biological Technology Pvt. Ltd. Wuhan, China).The quality of chromatograms was checked visually and sequence data were assembled and edited using SeqMan software (DNASTAR Inc., Wisconsin, USA). The assembled MLST-1 sequences were compared using non-redundant database (NRDB) comparison tool available in http://pubmlst.org/analysis/ with our previously analyzed 10 strains used as a control to assign allele and Sequence type number (Rosales e t al., 2015). MLST-2 scheme is based on a partial sequencing of adh-1, gltX, gpsA, gyrB, pta-2, tdk and tkt (Register et al., 2015). For MLST-2 scheme, the 44 Chinese strain and PG45 were subjected to PCR, and PCR products were sequenced as above mentioned method. The assembled sequences of all isolates were uploaded to http://pubmlst.org/mbovis/database to identify allele numbers and sequence types (STs). In addition, for the evolutionary assay, 8 Israel strains were typed with the method as described above. Meanwhile, 8 Australian isolates whole genome were annotated using prokka 1.11rapid prokaryotic genome annotation software (Seemann; 2014) at http://www.vicbioinformatics.com. Each locus sequence was extracted from the annotated genome. Pulsed Field Gel Electrophoresis (PFGE) analysis PFGE of 44 Chinese M.bovis field strains and type strain PG45 was performed as previously described (McAuliffe et al., 2004, Arcangioli et al., 2012) with some modifications for agarose block preparation. Briefly, macro-restriction analysis was performed with the restriction enzyme SmaI as follows: Each M.bovis isolate 15 ml culture aliquot was centrifuged at 15000 à ¯Ã¢â‚¬Å¡Ã‚ ´g for 20 min at 40C, the pellet was washed three times with Tris-EDTA buffer and resuspended in 400 à ¯Ã‚ Ã‚ ­l of cold Tris-EDTA buffer (pH 8.0). Agarose plugs were prepared from a 1:1 mixture of the above cell suspension and 2% low-melting-boiling agarose (Bio-Rad). They were then incubated in a lysis buffer containing 10mM Tris-HCl, 1 mM EDTA, 1% lauroyl sarcosine, 1mg of proteinase K per ml for 48 h at 560C. These plugs were washed for 6h with several changes of Tris-EDTA buffer at 40C. The plugs were then cut aseptically into 2 mm sections and equilibrated in 120 à ¯Ã‚ Ã‚ ­l restriction buffer (Prom ega) for 30 min at 40C. Subsequently, plugs were digested with 30U of SmaI (Promega, Shanghai, China) at 240C for 4 h. After digestion loaded in 1% pulsed-field-certified agarose gel (Bio-Rad), and run in a CHEF-DRIII system (Bio-Rad) at 6V/cm, in 0.5à ¯Ã¢â‚¬Å¡Ã‚ ´ TBE buffer at 140C, at 6V/cm with angle of 1200. The initial pulse time was 5s, with a final pulse time of 40s with a running time of 24 h. The lambda DNA ladder PFGE marker (Bio-Rad) was used as a reference. PFGE fragments in the gel were stained with ethidium bromide (EB) (1mg/ml) for 20 min, and destained in distilled water for 1.5 h and visualized under UV transilluminator. Pulsotypes (PT) were assigned numbers consecutively based on differences of more than one band in PFGE patterns upon visual inspection. The banding patterns were analyzed using Dice coefficients with 1% band position tolerance. The clustering of patterns was performed using unweighted pair group matching algorithm (UPGMA) as previously described ( Arcangioliet al., 2012; Timsit et al., 2012). Allelic sequence variance analysis The Sequence Type Analysis and Recombinational Test Version 2 (START2) (Jolley et al., 2001) were used to analyze polymorphic sites, construct UPGM dendrograms and calculate non-synonymous to synonymous ratios (dN/dS). Genetic diversity (H) of each locus and Index of Association (IA) were calculated by using LIAN 3.5 (Haubold and Hudson, 2000) hosted on http://guanine.evolbio.mpg.de/cgi-bin/lian/lian.cgi.pl/query. Global evolution and minimum spanning tree (MST) analysis The evolutionary relationship between isolates and M.bovis population structure was determined using PHYLOViZ (Fransciso et al., 2012) and evaluated by minimum spanning tree (MST) created using eBURST (geoBURST) algorithm (Francisco et al., 2009). MST for MLST-2 was performed for 257 isolates from 11 countries including 60 strains (44 China, 8 Israeli and 8 Australia isolates)   typed in this study and 207 isolates   retrieved January, 2017 (Supplementary Table 3) from the M.bovis MLST-2 database www.pubmlst.org/mbovis. Statistical analysis The discriminatory ability of both MLST methods and PFGE was calculated using Simpsons index of diversity as previously described (Hunter and Gaston, 1998). Congruence between both typing techniques was measured using the adjusted Rand Coefficient and Wallace Coefficient (Severiano et al., 2011). All statistical analyses were performed using the freely available online tool (http://darwin.phyloviz.net/ComparingPartitions/) Results The comparison of M.bovis typing with three methods MLST-1 analysis A total of 44, out of 10 were previously typed (Rosales et al., 2015) were also used for control and typed by MLST-1. The mean GC contents of seven gene fragments ranged from 29.15% (dnaA) to 37.23% (tufA) while it was 37.4 % in the whole M. bovis HB0801 genome (Qi et al., 2012). For each of seven loci, allelic variation was analyzed including polymorphic sites, guanine-cytosine(GC) content, synonymous and non-synonymousratios (dN/dS)(Table 2).The number of polymorphic sites per locus ranged from 4 (6.2%) in recA to 19 (29.6 %) in dnaA, and a total of 64 polymorphic sites for all seven genes were identified. The number of alleles observed ranged from 2 (metS, recA, tufA, atpA, and tkt) to 3 (dnaA and rpoD). The genetic diversity (H) for each locus was 0.0879 for dnaA and 0.0444 for metS, recA, tufa atpA and tkt. The dN and dS substitutions ranged from 0.0000 to 0.0623. In summary, all 44 Chinese M.bovis isolates typed by MLST-1 were divided into two STs namely ST-10 and ST-34 (Table 1).The ST-10 (with allelic profile of 2,6,2,2,2,5,3) was most numerically dominant, comprising 97.7%à ¯Ã‚ ¼Ã‹â€ 43/44à ¯Ã‚ ¼Ã¢â‚¬ °of Chinese M.bovis isolates including the Chinese strain HB0801 (Fig.1). In addition, ST-34 (allelic profile of 11,6,2,2,2,5,3) contains only one strain SZ; while ST-1(allelic profile of 1,1,1,1,1,1,1) represented by strain PG45 was identified (Table 1). Genetic relatedness amongst the 44 Chinese M.bovis strains showed two clades A and B. Clade A contained the majority (97.7%) of isolates (43/44) including the Chinese strain HB0801, while clade B contained one Chinese strain SZ (ST-34). M.bovis PG45 type strain was an outlier of these two clades (Fig.1). The geoBURST and MST analysis clustered 44 Chinese in the clonal complex CC2, whereas reference strain PG45 (ST-1) in CC1 (Table 1) as previously described (Rosales et al., 2015) MLST-2 analysis All 44 M.bovis isolates were examined by MLST-2. The mean GC contents of seven gene fragments ranged from 28.76% (tdk) to 35.61% (gyrB).The number of polymorphic sites per locus ranged from 8 in gyrB (8.66%) to 22(23.91%) in gpsA and a total of 92 polymorphic sites were identified (Table 2). The numbers of alleles identified were 2 for adh-1, gpsA, gyrB, pta2 and tkt and, 3 for gltX. The genetic diversity obtained 0.328 for adh-1 to 0.962 for gpsA (Table 2).   The Chinese strains were distributed into two different sequence types. ST-10 with allelic profile 4,3,3,3,5,3,4 was the most numerically dominant type, comprising 97.7% (43/44) of Chinese isolates; and ST-32 had only one isolate, SZ respectively. All M.bovis isolates tested in this study were clustered into two major clades A and B based on genetic relatedness by UPGMA. Clade A was comprised of 97.7% (43/44) of Chinese isolates including the Chinese strain HB0801. Whereas Clade-B contains one Chinese isolate. Same as above, M.bovis PG45 type strain was an outlier of these two clades (Fig. 2) PFGE typing The 44 Chinese M.bovis strains, and type strain PG45 were subjected to PFGE following the use of restriction enzyme SmaI. All isolates were typeable and the banding profile of the isolates ranged from 6 to 10 bands (from

Importance of Sports Essay

Sports such as football or baseball involve lots of physical activities. Sports and exercises help in strengthening and toning the muscles and bones in the body. In short, the importance of sports for kids is that it keeps them in an excellent shape. When children or adults plays team sports, be it cricket or hockey, they learn to work in groups. They learn that if the team wins, they win and if the team loses, they lose. This way they learn how to work in groups. Thus, the importance of sports for kids is that they understand what is team spirit and thus, when they grow and actually start working, it will help them immensely in building relationships with their co-workers, and also to work in harmony with others. Sports makes people mentally strong. Success and failure are both parts of sports as well as life. A sportsman knows that there will be times when he will win matches, there will also be times when he will lose them. A sportsperson knows how to handle defeat and thus, treats success and failure equally. This is an important life lesson too, which sports can teach a person. Besides this, another importance of sports for children or for adults is that it teaches them how to handle competition, and be fearless when facing the adversaries. Children and adolescents ooze with physical energy. When they are involved in sports, their physical energies are used up in a constructive way. Teenage is such an impressionable age, if adolescents are given free time they might get involved in wrong activities or may fall in bad company or may also display anti-social behavior. Thus, the importance of sports in society is that it keeps adolescents from becoming anti-social elements, who might otherwise disturb the delicate fabric of society. Here’s hoping that now you know what is the importance of sports. Besides being important for kids, taking up a sports career in adult life, has its own benefits. A sportsperson often travels to other countries to play matches and in the process, learns a great deal about the cultures of these countries. Even the spectators or TV viewers are thoroughly entertained while watching professional sports, making it an excellent recreational activity.

A Proposed Computerized Payroll System Essay

Chapter I INTRODUCTION Electricity is a naturally occurring force that exists everywhere and it is used to power many things that are used in our everyday life. Without electricity, people’s lives would be very different and in many cases more difficult. There are many ways to generate electricity; one way in producing electricity is the use of solar cells. Solar cells or photovoltaic cells are made of semiconductor materials such as silicon and designed to convert light energy into electrical energy by the photovoltaic effect. Photovoltaic effect is the basic physical process through which a solar cell converts light into electricity. Light is one of the most abundant forms of energies and by using this energy in a proper way an eco-friendly form of energy can be produced. When light energy strikes, it absorbs photons of light and releases electrons these free electrons then forms an electric current that can be used to power a load. According to The World Factbook, the world has over 5 billion mob ile phone users. In the Philippines alone, there are 92,227,000 mobile cellular telephone subscribers, placing the country in the 11th position of most number of mobile phone users in the year 2010. This fact shows that mobile phones are essential therefore the batteries are needed to be charged and one way to charge it, is through solar energy. For its commercial purpose, the solar powered mobile phone charger is designed to have a coin timer that will recognize the coin to activate the timer and the mobile phone charger. The time that it will take for the mobile phone to charge depends on the amount of money that will be inserted in the device. Through this, the materials used for the machine, like the solar calls, will be recovered. The convenience of the machine lies in its capability to be installed either indoor or outdoor locations as long as there is light that can be absorbed by the photovoltaic cells. Background of the Study From the survey given randomly to some students and employee’s most of them frequently use†¦. try dn to.. From the survey that was gathered, most people frequently usetheir mobile phones and sometimes forget to charge their phones at home; such causes them to run out of battery charge when they need  to use it outside, especially in emergency purposes. The places where they need to charge their phones the most in case of emergency are schools, hospital, mall, offices, bus stations and other terminals. For the past years, a mobile phone charger coin-operated machine already exists in the Philippines and this was stated from the article of Rey Gamboa from Philippine Star. However, this machine is usually available on public and indoor places like Ministop and 7-eleven and are powered by power grids. Therefore, the proposed design is a mobile phone charger vending machine that uses solar cells to produce electricity that will power the device without the need for outlet plugging(the power in the outlet†¦ try to†¦ ). It can be used on both indoors and outdoors as long as light is available.It may also payback the expense of the material used and the good thing is that the time span of a solar cell is about 30 years according to the studies done in 2007 by International Journal of Environmental Technology and Management. It may also payback the expenses for the materials used in having solar cells and it has a time span of more than thirty years according to the studies of the International Journal of Environmental Technology and Management in the year of 2007. trydn†¦ Statement of the Problem This project study dealt in the development of using free energy caused by light that produces electricity for the mobile phone charger. This form of transforming energy will help lessen the environmental problem such as pollution, global warming, acid rain or smog.At the same time, it paybacks the causeof the solar cells itself by using it as a solar powered mobile phone chargervending machine. The study specifically aims to answer the following questions: 1. What type and how many solar cells are needed to produce electricity that is enough to power the mobile phone charger vending machine? 2. How to design the power supply circuit necessary to power the mobile phone charger vending machine? 3. What is the position and alignment of the solar cells and also the time in which it can produce electricity efficiently? 4. How to design and develop the mobile phone charger circuit that can accommodate 2 units of mobile phones? 5. Howlong is the payback time in using solar cells to power t he mobile phone charger vending machine? Objectives of the Study The researchers’ main objective is to design and develop a solar powered mobile phone charger vending machinethat can maximize the profit of the investors that operates it. This means that this device can be installed in any areas as long as the solar cells can be exposed to sunlight which will eliminate the need of an outlet.In order to address the main objective, the following also need to be achieved: 1. To determine the type and number of solar cells needed to produce electricity that is enough to power the mobile phone charger vending machine. 2. To design the power supply circuit necessary to power the mobile phone charger vending machine. 3. To determine the position and alignment of the solar cells and also the time in which it can produce electricity efficiently. 4. To design and develop the mobile phone charger circuit that can accommodate 2 units of mobile phone. 5. To determine thepayback time in using solar cells to power the mobile phone charger vending machine. Significance of the Study People who are using mobile phones frequently are the main recipients of the benefits in this study. The need of people to charge their mobile phone because of work, emergency, or any other condition will lead them to find a mobile phone charging station. Hence, a mobile phone charging station that is powered by light energy will be a good way to harness energy from light efficiently and productively. For the environment, the device uses only the photons of light to produce electricity which means that it doesn’t need to be plugged into a power source. It can also be used inside anenclosed area where there is artificial light like bulbs or fluorescent light. However, for higher gain of electricity and efficiency it is recommended to harness sunlight’s energy because it is brighter than any other artificial light that is common in commercial and public areas. For the investors, after the device is produced, it in itself will produce electricity for it to work. Consequently,the device doesn’t need intensive maintenance, and the cost of maintenance will be less. For the future researchers, energy sources has so many forms; the way of harnessing it to its’ full potential is the only difference. This device is an example of harnessing light energy in a way that will help the producer, consumer and environment. This will help future researchers to think of topics that will not only benefit one party, but all  those who are going be involved or affected by their proposed project. For the researcher, this device is another innovation in the field of harnessing solar energy. This simply shows that solar energy can be used as an alternative source of energy in many ways. Scope and Limitations of the Study The researchers’ main concern is to design a solar powered mobile phone charger vending machine that is efficient on harnessing solar energy and can convert it to electrical energy to charge mobile phones. The device must be economically sound in a way that when it has achieved its payback period, the only thing that must be put into concern is the cost of maintenance. Since solar cells are easy to maintain, the maintenance cost is not high. The devices’ main part is the power supply that will act as the source of electricity for the device. Solar cells effective life span is 25-30 years, which makes it a very good alternative source of energy for the device to work. To design the devices’ main part, the position and slope of the solar cells must be considered depending on the location where it will be installed so that the solar cells can produce the maximum amount of electricity. This is because the amount of electricity that a solar cell can produce depends on the intensity of sunlight that is being absorbed within the semiconductor material. The number of solar cells must also be enough to produce electricity that is needed to power the device. The rated total power output of all the solar cells must also be sufficient for the mobile phone vending machine. It must also be placed on top of a roof or any high position so that shadowing, vandalism and stealing can be prevented. A battery will serve as a storage device so that the electricity that will be generated by the solar cells will be stored whenever it is not in use and the electricity that will charge the mobile phones will be constant and not fluctuate. The power supply circuit will regulate the voltage and current to its rated value that the mobile phone charger vending machine needs. The devices’ second main part is the mobile phone charger vending machine. This is composed of a mechanism that recognizes the coin that is inserted and starts the process of charging the mobile phone and also the timer. It recognizes the diameter, thickness and weight of the coin so that it can distinguish the value of the coin. The timer is programmed to start whenever a coin is inserted and it depends on the value of the coin that is inserted  on how many minutes the timer will countdown. At the same time, the charger will start charging the mobile phone until the timer ends the countdown. The devices’ third main part is the mobile phone charger circuit. This charger can charge multiple mobile phones at the same time. It is composed of different plug-ins that is commonly used for the user to choose of the plug-in that fits in his/her mobile phone. It is operated by the timer so when the timer starts to countdown, it starts to get electricity from the power supply. Theamount of voltage and current output that is needed to charge the mobile phones is suitable for nearly all brands of mobile phones. Limitations of the study The device is subjected to the following limitations: 1. In outdoors, the device only operates during daytime, approximately 6:30am – 5:30pm depending on the location as long as sunlight is available. 2. It can also be used inside a mall or commercial area that has artificial lighting system,however the amount of electricity that the solar cell can produce is not as high compared to what sunlightcan produce. 3. The plug-in that will be used to charge the mobile phones are those of Nokia. 4. Since the device only recognizes 1Php and 5Php, it can only be used in the Philippines. 5. It cannot be used outdoors when sunlight is not present specifically during rainy days or in cases when the clouds are very gloomy. 6. It cannot be used outdoors when there is a natural catastrophe or disaster. 7. The researchers will not consider the charging time of the Deep cycle battery. 8. The researcher will not consider if the mobile phone user doesn’t ends the countdown of charging his/her mobile phone.

Addiction A Serious Problem Essay - 1559 Words

Addiction is a very serious problem in today’s society. It is the goal of counselors to help those who suffer from addictions. There are many different models that attempt to explain what addiction is, and how someone gets addicted. There many different views about addiction. â€Å"Historically addiction has been understood in various ways- a sin, a disease, a bad habit-each a reflection of a variety of social, cultural and scientific conceptions(Hammer et al., 2012 p. 713). While there are many different models of addiction three of the most common models are The Disease model, The Genetic Model, and the Family model. All of these models view addictions differently and have a different perspective on how to treat the addiction. The disease model of addiction is one of the best known model of addiction. The disease model views addiction as an illness that the addicts has. â€Å"The disease concepts follows the medical model and posits addiction as an inherited disease that c hemically alters the body in such a way that the individual is permanently ill at a genetic level† (Lee et a., 2013, p 4).This is the model that groups this Alcoholics Anonymous adopts to help its clients. This is a good model to explain why someone is addicted. It shows that the addiction is an illness that the person needs to fight. The way that a person fights this disease is to get help and to abstain from the substance. However there are some issues with this model. The first being that this model viewsShow MoreRelatedDrug Addiction Is A Serious Problem1032 Words   |  5 Pages Drug addiction is a serious problem in today’s society. Drug addiction is a complex disease and once addicted, it is nearly impossible to quit. 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Do we reach for our smartphone first thing in the morning? Is our smartphone the last thing we check at night? In â€Å"Stop Smartphone Insanity†, the purpose of the commercial is to persuade people to stop smartphone addiction that i s dangerousRead MoreDrug Abuse1279 Words   |  6 PagesThe use of and abuse of illegal and prescription drugs are a health, social, and law enforcement problem that is affecting Americans across the country. Drug abuse is destroying the lives of many teens and adults and is also destroying families in the United States. The use of drugs is a major problem in the United States among all Americans, but drug addiction is the main cause for America s troubled teens today. Exactly what is a drug? A drug is any chemical that produces a therapeutic or non-therapeuticRead MoreGambling Essay1227 Words   |  5 Pagesvarious addictions in the world today such as, drug, alcohol, sex, eating, or gambling addictions. One might ask the question, is one addiction more serious than another or are all addictions equally destructive? In particular, is an addiction such as gambling as serious as an addiction to drugs or alcohol? Research suggests a gambling addiction is less severe than a drug or alcohol addiction because drug or alcohol addictions are psychological and physical, can cause other addictions, can resultRead MorePhone Addiction Essay713 Words   |  3 Pageshealth in multiple ways. Phone addiction is a serious problem that needs to be solved. Using just a few simple techniques you could identify and solve your phone addiction. The basic steps to solving your phone addiction are, identifying your addiction, challenging yourself, and working with f riends. Phone addiction is a big problem world wide. Loss of sleep can be caused by the lights that are coming out of the phone screen, which can lead to many health problems. There has also been a correlationRead MoreHow Smartphones Affect The Growth Of Bad Habits1167 Words   |  5 Pagesare addicted to their smartphones, while more than half of parents feeling that their kids’ addiction (TeenSafe). Smartphone has already been an inseparable part of many people’s daily life. Just like everyone around them, teens and kids are using and becoming more and more dependent on the smartphones. Problems are different among the different audience. When it comes to teenagers, this could be a serious one. Overall, teenagers are at an important growing stage in their life. The main characteristicsRead MoreThe Effects Of Video Game Addiction On Human Body And Mind1151 Words   |  5 PagesVideo game addiction â€Å"generally refers to an excessive, unhealthy amount of playing of games. Rather than engaging in the real world, an addicted user devotes the majority of his or her time to gaming. The addicted gamer often isolates him/herself from others, ignores more important responsibilities, and is often obsessed with obtaining higher status / ranking / achievements in his/her favorite game.† (Conrad, page 1, par.1) This is an uprising problem between teens and adults that is forming intoRead MoreHow Addictions Destroy Family Unit990 Words   |  4 PagesHOW ADDICTIONS DESTROY THE FAMILY UNIT In our modern life, there are certain things that can destroy the family unit queitly such as addictions. An addiction is anything that one must have in order to avoid a negative feeling or syptoms. Addictions can include almost anything, not just foods, drinks or other physical substances. Some addiction are mend to make one calm, but it easily becomes an emotional crutch that unfortunately usually worsen physical aspects of addiction. However, the point